Tumor cells are highly adaptive and can optimize their metabolism for proliferation, build-up of biomass and survival under conditions of stress.
We are particularly interested in the metabolic adaptations that allow tumor cells to survive in hypoxic areas and in monitoring possible effects of anti-angiogenic treatment in glioblastomas. In cooperation with other laboratories, we analyse the metabolites extracted from tumor tissues using different mass spectrometry based methods (GC-MS, LC-MS). Furthermore, we are in the process of mapping the human GBM metabolome using 13C6-glucose metabolomic flux analysis in patients.
The figure below shows the strategy for mapping the human GBM metabolome. Patients are infused with 13C6-glucose, before surgery. Under surgery defined biopsies are selected for LC-MS metabolic flux analysis.